Reference material is a substance (one or more) of whose characteristics are significantly homogenous and adequately established to be utilized for the calibration of an element. The measurement as well as assessment techniques or for assigning values to the materials.

Most of the reference materials which are currently accessible do not stand for the matrices in which oceanographers work, and they also do not have the desires analytes at similar concentrations as ones found on nature. Moreover, the sources of the reference materials are endorsed. They are also supposed to be diverse, and details about them should be well-coordinated like for herbal reference.

The following are the conditions for the preparation of reference materials which will be mutually acceptable to firms all across the world;

Homogeneity: this makes sure that the analysis of every subsample of the content taken to be measured will offer some analytical outcome within the stipulated measurement uncertainty.

Stability: the manufacturers are supposed to show how long the reference material can have useable life because they tend to be sensitive to time, light, microbial activity, and temperature, among others.

Correspondence to the real sample: to manufacture significant analytical outcomes, the reference material is supposed to have similar characteristics as much as possible as the matrix of the sample.

Ambiguity, traceability, and exactitude: a licensed value is the ideal approximation of the real and accurate concentration of the analyte.

Preparation of a reference substance

  • Give details of the reference material such as the matrix, its characteristics to be endorsed, as well as their desired amounts
  • Process of design sampling
  • Preparation of the design sample
  • Choose a method suitable stability testing and homogeneity
  • Show the properties of the reference materials
  • Obtain the samples
  • Prepare the samples
  • Homogeneity testing
  • Stability testing
  • Illustrate the reference material
  • Syndicate your findings from the stability as well as the homogeneity tests and the illustration and come up with an uncertainty statement
  • Develop a certificate if needed, a certification report

All reference materials need to be sourced from credible sources since it is imperative for laboratories to have confidence and trust the competence of the manufacturers from where they get the reference materials.